immunofluorescence reagents
  • National Day Holiday Announcement
    National Day Holiday Announcement September 30, 2021
    This is an official announcement for the National Day of the People Republic of China.   All our clients are informed that we will close on Friday 1st October and will resume business as usual on Wednesday 8th October on account of the National Day holiday. We pay homage to the founding of our nation and the valiant soldiers who laid down their lives for this country.   National Day holiday: 1st October-7th October 2021 Reopening date: 8th October 2021 During the time, if you have questions regarding the business, please feel free to call the office.   Congratulations and Thank You Everyone for Contributing to The Success of Our Enterprise.   Xiamen Biotime Biotechnology Co., Ltd 30th September, 2021
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  • Chemiluminescent Immunoassay
    Chemiluminescent Immunoassay September 02, 2021
    What is Chemiluminescent Immunoassay Chemiluminescent immunoassay (CLIA) is an immunoassay technique where the label, i.e. the true “indicator” of the analytic reaction, is a luminescent molecule. In general, luminescence is the emission of visible or near-visible radiation which is generated when an electron transition from an excited state to a ground state. The resultant potential energy in the atom gets released in the form of light. (Cinquanta, Fontana and Bizzaro, 2017) Chemiluminescent immunoassays offer one of the best solutions for the quantification of low concentrations of specific analytes from a complex mixture for in vitro diagnostic industry. They can also be used as diagnostic tools in medicine, as well as being used in several other different industries for various applications. (Lorne Laboratories, 2021) How Does Chemiluminescence Work Chemiluminescence immunoassay (CLIA) is an assay that combines the chemiluminescence techniques with immunochemical reactions. The assay format is similar to enzyme-linked immunoassays, usually based on heterogeneous assays where antibodies or antigens are immobilized on a solid phase but one of the components is conjugated with a chemiluminescent label. (Lorne Laboratories, 2021) (BrightTALK, 2021) CLIA utilizes chemical probes which could generate light emission through a chemical reactions to label the antibody. (Creative-diagnostics, 2021) The Benefits of Chemiluminescence Immunoassay In recent years, CLIA has gained increasing attention in different fields, including life science, clinical diagnosis, environmental monitoring, food safety, and pharmaceutical analysis because there are benefits of using chemiluminescence such as: ultra-sensitive good specificity wide range of applications simple equipment wide linear range easier in operation and design can detect small amounts of the biological molecule does not require long incubations and the addition of stopping reagents (Creative-diagnostics, 2021) Key Types of Chemiluminescent Immunoassay Methods Chemiluminescent methods that have been commonly seen are direct - using luminophore markers, indirect—using enzyme markers, or electrogenerated – typically nanomaterials based. (Cinquanta, Fontana and Bizzaro, 2017) (Ju, Lai and Yan, 2017) Indirect CLIA, using enzyme markers This common CLIA method involves an enzyme, typically horseradish peroxidase (HRP) or alkaline phosphatase (AP), conjugated to a secondary antibody. This HRP– or AP–secondary antibody complex then catalyzes the conversion of chemiluminescent substrate, luminol and CSPD reagent respectively. These substrates, when oxidized, provide an easy means of signal detection and quantification due to prolonged signal emission. (Shimadzu, 2021) Direct CLIA, using luminophore markers This method commonly uses acridinium esters as luminophores conjugated directly to the primary monoclonal antibody to generate a signal. Acridinium esters are highly specific labels that spontaneously oxidiz...
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  • Biotime HPLC analyzer// Gold standard for HbA1c measurement
  • [Biotime announcement] - Biotime infection marker interleukin 6 (IL-6) is officially launched!
    [Biotime announcement] - Biotime infection marker interleukin 6 (IL-6) is officially launched! April 22, 2021
    Interleukin is a type of cytokine produced by and acting on a variety of cells. Interleukin 6 (Interleukin 6, abbreviated IL-6) is an important member of the cytokine network, and it has been widely used in many clinical fields. PCT has been widely recognized clinically in differential diagnosis of bacterial infection, risk stratification of sepsis and guidance of antibiotic use. IL-6 is an important cytokine expressed in the initial response of the innate immune system to injury and infection, and plays an important role in the early diagnosis of acute infection. IL-6 combined with PCT has complementary advantages, improves detection sensitivity and specificity, and better facilitates the management of the whole course of sepsis. IL-6 is also one of the important monitoring indicators during the diagnosis and treatment of COVID-19. IL-6 is used for early warning of sepsis After the occurrence of inflammation, IL-6 is the first inflammatory marker. IL-6 will be involved in the occurrence and development of many diseases, and its blood level is closely related to inflammation, viral infection and autoimmune diseases, and its changes are earlier than CRP and PCT. Studies have shown that IL-6 increases rapidly after bacterial infection, PCT increases after 2h, and CRP increases rapidly after 6h [1]. So IL-6 can be used for early warning of sepsis. (Kinetic changes of inflammatory markers in vivo after endotoxin stimulation) IL-6 is used to evaluate the prognosis of sepsis Studies have shown that the high IL-6 concentration during the treatment of sepsis indicates the poor prognosis of patients and the higher mortality rate at the later stage of treatment. The expert consensus on the interpretation of the clinical significance of infection-related biomarkers mentioned that IL-6> at 1000 pg/mL suggests a poor prognosis. Therefore, the detection of IL-6 is also of great significance for the prognosis assessment of sepsis. Application of IL-6 in COVID-19 IL-6 is an important pathway that induces cytokine storm. Cytokine storm is a kind of transitional immune response, and the uncontrolled release of inflammatory factors eventually leads to organ damage and even functional failure. From a large number of research data, it is generally believed that cytokine storm is a key factor in the deterioration of COVID-19 patients. Therefore, IL-6 is also an important monitoring indicator in the diagnosis and treatment of COVID-19, which can be used for early warning of severe/critical COVID-19 and can also help guide the clinical selection of appropriate treatment strategies. a) Key laboratory indicators for COVID-19 patient assessment b) IL-6 levels in 99 confirmed COVID-19 patients Figure (a) In a retrospective multicentric study of 150 confirmed COVID-19 cases, IL-6 in the deceased patients was significantly higher than that in other cases [2]. Figure (b) In a study of 99 patients published in the January 29 Lancet, IL-6 was significantly elevated in more th...
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  • Biotime attended medical trade fair CACLP 2021
    Biotime attended medical trade fair CACLP 2021 March 31, 2021
    Established in 1991, CACLP Expo now is the largest, the most influential and professional trade show in China In Vitro Diagnostics (IVD) and clinical laboratory industry. Biotime, a leading manufacturer of POCT, attended China Association of Clinical Laboratory Practice (CACLP) Expo 2021 from March 28th to 30th at Chongqing International Expo Center. During this CACLP, Biotime exhibited a full line of Point of Care Testing (POCT) reagents and released 3 automated analyzers, named HLC-100 Fully Automated HbA1c Analyzer, FLI-600 FIA Analyzer and FLI-4000 Fully Automated FIA Analyzer. At the same time, Biotime brought a wonderful academic satellite symposium. Cardiology expert Wang Bin was invited to introduce the chest pain center and related cardiac markers. Biotime’s new innovations attracted lots of medical experts and distributors' attention to stop by and communicate with each other. We are so grateful for visitors' support and wish to meet you next year!
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  • Product Recommendations | NGAL- Early Marker of Acute Kidney Injury
    Product Recommendations | NGAL- Early Marker of Acute Kidney Injury July 05, 2021
    What is NGAL? The full name of NGAL is human lipocalin 2 (Ln2) or siderocalin. It belongs to the lipocalin family and is the earliest proposed marker of acute kidney injury (AKI).   What is the clinical significance of NGAL? 1. NGAL is a marker of acute kidney injury (AKI) When AKI occurs, NGAL rises rapidly in a very short period of time, and the most obvious is 2h. Serum creatinine (sCr), urinary enzymes, etc. often increase significantly after 24 to 72 hours. At this time, the glomerular filtration rate (GFR) has decreased significantly, and NGAL can change before GFR decreases, so it is suitable for early diagnosis of AKI. At the same time, the increase in NGAL concentration is closely related to the duration of AKI, the severity of kidney injury (RIFLE classification), and the treatment effect and prognosis. 2、Can predict the progression of chronic kidney disease (CKD), especially diabetic nephropathy Studies have found that NGAL may be involved in the occurrence and development of diabetes as an inflammatory factor. In diabetic nephropathy, renal tubule damage may be earlier than glomerulus. As a marker of early renal tubular damage, NGAL can reflect renal function damage in diabetic patients (especially type 2 diabetes) before urinary microalbumin (mALB). 3、NGAL also has a certain relationship with tumors Data from different laboratories indicate that the concentration level of NGAL is related to a variety of tumors. It promotes cell proliferation by uptake of iron ions and helps tumor cell growth and infiltration. It is related to tumor cachexia and has become an independent predictor of poor prognosis for patients with cachexia. Compared with traditional projects, what are the advantages of NGAL? When AKI occurs, NGAL rises rapidly in a very short time. The following figure shows the time of increase in the concentration of various markers after the occurrence of AKI: Therefore, testing NGAL can buy more time for AKI patients and grasp the best time for treatment. What are the clinical applications of NGAL? Due to the excellent predictive and diagnostic value of NGAL in AKI, the detection of NGAL can be increased in clinical situations that easily cause AKI. · Routine monitoring after major surgery · Monitoring of ICU patients · Triage of conditions in the emergency department · Early diagnosis of AKI caused by infection and sepsis · Contrast nephropathy (CIN) monitoring after interventional therapy...... NGAL is also an evaluation index of chronic kidney disease, so it can also be used to predict the progression of chronic kidney disease and early detection of kidney damage in patients with diabetic nephropathy. The neutrophil gelatinase-associated lipocalin assay kit provided by Biotime uses fluorescence immunochromatography, which is easy to operate and results in 10 minutes. Product Name:NGAL Rapid Quantitative Test Link: https://www.xiamenbiotime.com/ngal-rapid-quantitative-test_p60.html
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  • What you should know about D-Dimer?
    What you should know about D-Dimer? June 16, 2021
    D-Dimer is one of the biomarkers with high attention on the market in recent years, which is used to indicate thrombosis and thrombolytic activity in human body. Let’s first talk about its clinical application. 1. Venous Thromboembolism (VTE) the level of D-Dimer is significantly increased in VTE patients, however, some diseases such as cardiovascular disease, surgery, tumor, infection, and tissue necrosis also have a certain impact on blood clotting, which cause D-Dimer to increase. Therefore, the sensitivity of D-Dimer is 97%, but the specificity is around 61%-64%[1]. So D-Dimer can not be used to diagnose VTE alone. The practical significance of D-Dimer is that a negative result can exclude VTE. 2. Deep vein thrombosis (DVT) a blood clot that's deep inside a vein. These clots usually affect the lower legs, but they can also happen in other parts of the body. 3. Pulmonary embolism (PE) a blockage in an artery in the lungs. It usually happens when a blood clot in another part of the body breaks loose and travels to the lungs. DVT clots are a common cause of PE. 4. Disseminated intravascular coagulation (DIC) DIC is a syndrome characterized by the systemic activation of blood coagulation, which generates intravascular fibrin, leading to thrombosis of small and medium-sized vessels, and eventually organ dysfunction. DIC may occur as a complication of infections, solid cancers, hematologic malignancies, obstetric diseases, trauma, aneurysm, and liver diseases, etc. International Society on Thrombosis and Haemostasis(ISTH) suggested that a reduced fibrinogen level is valuable for the diagnosis of DIC, but is not observed in most DIC patients. The elevated fiber-related markers (FRMs), such as fibrinogen and fibrin degradation products, D-dimers, and soluble fibrin (SF), reflect thrombin formation[3]. 5. D-Dimer and COVID-19 Correlations exist between COVID-19 infection, severe elevation of D-dimer levels, and increase in the rate of complications and composite end-point. The appropriateness of early and continuous D-dimer monitoring and labeled anticoagulation as management tools for COVID-19 disease deserves accurate investigation, to prevent complications and reduce interventions[4]. Chinese Clinical Guidance for COIVD-19 Pneumonia Diagnosis and Treatment(7th edition) recommended that D-Dimer can be used for the diagnosis of severe COIVD-19 patients. What is the best test method in detecting D-Dimer? ① Quantitative Test ② High specificity and negative predictive value ③ Good repeatability ④ The coefficient of variation is low when the result is at the critical level ⑤ Rapid and convenient What could cause D-Dimer false negative? ① The volume of the thrombus is small or the distal small thrombus ② Fibrinolytic activity is decreased ③ Unreasonable design of reference interval and threshold ④ There are problems with sample preparation In summary, a rapid, convenient, and quantitative D-Dimer test with high sensitivity has been widely u...
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  • COVID DELTA VARIANT: WHAT WE KNOW
    COVID DELTA VARIANT: WHAT WE KNOW July 09, 2021
    More than 18 month since the outbreak, COVID 19 is still raging in much of the world. The novel coronavirus has mutated into strains such as the Delta variant which was first detected in India and accounted for 99% of Covid-19 cases in the UK by mid-Jun, according to Public Health England. (The Guardian, 2021) Delta strain is a highly contagious and possibly more severe SARS-CoV-2 virus strain. Delta is spreading 50% faster than Alpha, which was 50% more contagious than the original strain of SARS-CoV-2—making the new variant 75% more contagious than the original. (YaleMedicine, 2021) And like we’re seeing now, Delta is outcompeting everything else and becoming the dominant strain. The symptoms of the Delta variant appear to differ from traditional COVID symptoms, According to data from the United Kingdom, the most common COVID symptoms may have changed from those we traditionally associated with the virus. Those include: Put aside fever and cough which have always been common COVID symptoms, and headache and sore throat which have traditionally presented for some people, a runny nose is now being reported significantly more frequently. Meanwhile, loss of smell, which was originally quite common, now ranks ninth. (Herrero, 2021) Although it's still not clear whether it it's more dangerous, scientists are becoming more confident the Delta variant represents a more transmissible SARS-CoV-2 strain. (Fox, 2021) What’s even more concerning, is that the Delta variant that has caused devastation in countries like India and the UK has now mutated to produce another variant called Delta Plus. (Joi, 2021) While vaccines are rolling out, not all of us are able to receive them as soon as we'd like. And because not all of us are yet fully protected, get tested if you have any symptoms, even if it’s “just a sniffle”, to protect yourself with some added peace of mind as well as others in your community. Through the course of the pandemic, Biotime’s consolidated its commitment to bringing accessible COVID-19 rapid self-testing to the masses. Hence a wide range of covid 19 test kits has been rolled out and made available both nationwide and worldwide. In particular, the BIOTIME SARS-CoV-2 IgG/IgM Rapid Qualitative Test is a lateral flow immunoassay intended for qualitative detection and differentiation of IgM and IgG antibodies to SARS-CoV-2 in human serum, plasma (potassium EDTA), and potassium EDTA venous whole blood. Our rapid antigen covid test kit is also qualified to detect both asymptomatic and symptomatic COVID-19, helping society collectively return to daily life. In addition to that, some scientific researches have purposefully assessed the use of D-dimer, PCT, CRP, IL-6, Ferritin as biomarkers for disease severity and mortality in COVID-19 patients. And the results are rather convincing to suggest they can provide important information on the prognosis of the novel corona virus infection. Below is Biotime’s product specification. Shop now on our websit...
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