Immunofluorescence Platform Mar 29, 2021
Immunochromatography is also called immunoaffinity chromatography. The specific processes are as follows: a specific antibody containing the marker is dropped on the bonding pad, and a specific antibody is added at a location of the nitrocellulose membrane. After the sample liquid of the sample to be tested is added to the sample pad, through the action of the capillary tube, the sample solution and the marker are uniformly diffused at the binding pad through the action of the capillary tube. The sample solution reacts with the europium chelate to form the complex of the europium antibody chelate. The complex containing the europium antibody chelate then flows forward. When the complex moves to the detection band, because the detection band contains a specific antibody, it has a specific immune reaction with the europium antibody chelate-containing antigen and finally forms the immune complex of antigen-antibody-europium antibody complex. At the same time, the labeled antibody was also captured on the test line and stayed in the test line, while the redundant markers continued to flow forward and were adsorbed to the control band by the protein antibody on the membrane, and the complex specific binding occurred on the control line, and at the same time, the labeled antibody was also captured on the control line. According to the difference of marker types, the signal is detected by the instrument.

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