25(OH)-D is a prehormone that is produced in the liver by hydroxylation of vitamin D3 (cholecalciferol) by the enzyme cholecalciferol 25-hydroxylase. Physicians worldwide measure this metabolite to determine a patient's vitamin D status.
Cardiac markers are used in the diagnosis and risk stratification of patients with chest pain, suspected Acute Coronary Syndrome(ACS) and Heart Failure(HF), etc.